Figure 1.
Opening of KCa channels is required for hCG-stimulated steroidogenesis in human GCs. Blocking of IK (1 μM TRAM-34) and SK channels (100 nM apamin) significantly attenuated hCG (10 IU/ml)-induced progesterone production (PANOVA = 0.0038). According to Newman-Keuls multiple comparison test only the hCG-treated group (*) is significantly different (P < 0.01 compared to all groups), whereas there is no difference between all other groups (P > 0.05). Data represent means ± SEM (n = 3 experiments using independent cell preparations treated on the 3rd day of culture for 24 h).