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. Author manuscript; available in PMC: 2010 Mar 12.
Published in final edited form as: Neuron. 2009 Mar 12;61(5):721–733. doi: 10.1016/j.neuron.2008.12.025

Figure 6. Neuromuscular synapses form in the absence of AChR prepattern.

Figure 6

20-somite stage (A-B, G-H) or 27 hpf (C-F and I-J) embryos after heat shock treatment. (A, B) Tg(hsp70l:UnpSV1-myc;unplugged) embryos received heat shock from the 10- to 20-somite stage, which rescued AChR prepattern. (C, D) Similar heat shock treatment (10-somite to 27 hpf) also restored motor axon pathfinding, but not neuromuscular synapses. (E, F) The same heat shock treatment rescued motor axons and neuromuscular synapses in Tg(hsp70l:UnpFL-myc; unplugged) embryos. (G, H) In contrast, heat shock between the 10- and 20-somite stage failed to rescue AChR prepattern in Tg(hsp70l:UnpFL-myc;unplugged) embryos. (I, J) Heat shock treatment of same embryos between the 26-somite stage and 27 hpf, i.e. after the time period of prepatterning, was sufficient to rescue neuromuscular synapses. Scale bars: 50 μm.