8.

Oral administration of diosmin reduces Aβ pathology in Tg2576 mice. Brain homogenates and sections from Tg2576 mice treated with 0.05% diosmin supplemented diet (n= 10) or control diet (n= 10). For (A), half-brain coronal sections were analysed by Aβ antibody 4G8 staining. For (B), percentage of 4G8 positive plaques (mean ± S.E.M.) was quantified by image analysis. A t-test for independent samples revealed significant differences (P < 0.001) between groups. For (C), total soluble and insoluble Aβ_{1–40, 42} peptides from homogenates were analysed by ELISA. For Aβ ELISA, data are represented as picograms of peptide present in milligrams of total protein. Diosmin treatment results in markedly reduced total soluble and insoluble Aβ_{1–40, 42} levels, 37% and 46%, respectively. A t-test for independent samples revealed significant differences (P < 0.005) between groups.