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. 2009 May 1;4(5):e5428. doi: 10.1371/journal.pone.0005428

Figure 3. Inhibition of translation elongation in mice bearing Pten+/−Eμ-Myc lymphomas leads to reduced amounts of Mcl-1, Cyclin D1, and c-Myc.

Figure 3

Mice bearing Pten+/−Eμ-Myc tumors were treated for 28 hrs (two injections; 24 hrs apart) with either Rap (4 mg/kg), HHT (0.25 mg/kg), Bru (0.5 mg/kg), Did B (0.05 mg/kg) or CHX (12.5 mg/kg). A. At 28 hrs (lanes 2–6) tumor cells were extracted, lysed, and proteins fractionated by SDS-PAGE followed by Western blot analysis. The vehicle control mice were treated like the 28 hrs compound treatments except that compounds were omitted. B. Assessment of mRNA levels in Pten+/−Eμ-Myc tumors by qRT-PCR. The change in mRNA amounts relative to vehicle-treated controls (V) from three independent experiments is shown as a bar graph with standard deviation. Quantitation of the data from three independent experiments performed as in A is also superimposed on these graphs.