Figure 3.

In vitro interaction studies between the various peptide elements of CHL D and CHL D, CHL I and CHL H. (A) The in vitro translated (IVT) subunits of the Mg-chelatase CHL H, CHL D, and CHL I. On each track of the SDS/PAGE 20% of the protein subunits used for the interaction assays (B and C) were separated. (B) The results of interactions between the fusion proteins GST-CHLD(N), GST-CHLD(NM₁LM₂), GST-CHLD(M₁LM₂), GST-CHLD(M₁LM₂C) (see Table 1), and the in vitro-translated subunits CHL H, CHL D, and CHL I. (C) The interaction between GST and the in vitro-translated subunits CHL H, CHL D, and CHL I.