Table 1.
The recombinant plasmid constructions used in this work
| No. | Construct | Plasmid | CHL D fragment | Cloning sites |
|---|---|---|---|---|
| 1 | GST-CHL D(N) | pEGKT | 63-374 | BamHI/HindIII |
| 2 | GST-CHL D(NM1LM2) | pEGKT | 63-485 | BamHI/HindIII |
| 3 | GST-CHL D(M1LM2) | pGEX4T-1 | 375-485 | BamHI/EcoRI |
| 4 | GST-CHL D(M1LM2C) | pEGKT | 375-748 | XbaI/SalI |
| 5 | CHL D | pGEM-7Zf(−) | 63-748 | Xbal/BamHI |
| 6 | CHL I | pGEM-7Zf(−) | ChlI | XbaI/HindIII |
| 7 | CHL H | pCR II | ChlH | — |
| 8 | BD-CHL D(N) | pAS2-1 | 63-374 | EcoRI/BamHI |
| 9 | BD-CHL D(NM1) | pAS2-1 | 63-398 | EcoRI/BamHI |
| 10 | AD-CHL D(NM1) | pACT2 | 63-398 | BamHI/EcoRI |
| 11 | BD-CHL D(NM1LM2) | pAS2-1 | 63-485 | EcoRI/BamHI |
| 12 | AD-CHL D(NM1LM2) | pACT2 | 63-485 | BamHI/EcoRI |
| 13 | BD-CHL D(M1LM2) | pAS2-1 | 375-485 | EcoRI/BamHI |
| 14 | AD-CHL D(M1LM2) | pACT2 | 375-485 | BamHI/EcoRI |
| 15 | BD-CHL D(M1LM2) | pACT2-1 | 375-748 | EcoRI/BamHI |
| 16 | AD-CHL D(M1LM2C) | pACT2 | 375-748 | BamHI/EcoRI |
| 17 | BD-CHL D(M2C) | pAS2-1 | 448-748 | EcoRI/BamHI |
| 18 | AD-CHL D(M2C) | pACT2 | 448-748 | BamHI/EcoRI |
| 19 | BD-CHL D(C) | pAS2-1 | 486-748 | EcoRI/BamHI |
| 20 | (M1LM2C)CHL D-lexA | pFBL23 | 375-748 | EcoRI/BamHI |
| 21 | BD-CHL I-CHL D(M1LM2C) | pAS2-1 | 375-748 | BamHI/SalI |
| 22 | CHL I | p423TEF | ChlI | SmaI/SalI |
| 23 | AD-CHL D | pACT2 | 63-748 | SmaI/EcoRI |
| 24 | AD-CHL I | pACT2 | ChlI | SmaI/XhoI |
| 25 | AD-CHL H | pACT2 | ChlH | SmaI/XhoI |
| 26 | BD-CHL D | pAS2 | 63-748 | SmaI/SalI |
| 27 | BD-CHL I | pAS2 | ChlI | SmaI/SalI |
| 28 | BD-CHL H | pAS2 | ChlH | SmaI/SalI |