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Purification of recombinant NtCP56 and processed protein. Protein samples were subjected to SDS-polyacrylamide gel electrophoresis under reducing conditions and stained with Coomassie Brilliant Blue R-250. Lane M, molecular mass markers with the sizes shown on the left in kDa. Lane 1, induced BL21 (DE3)+PET30a whole cell lysate. Lane 2, induced BL21 (DE3)+pET30a/NtCP56 whole cell lysate. Lane 3, 43 kDa purified recombinant NtCP56. Lane 4, 33 kDa processed mature recombinant NtCP56.
