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. Author manuscript; available in PMC: 2010 May 1.

Published in final edited form as: Exp Hematol. 2009 May;37(5):549–558. doi: 10.1016/j.exphem.2009.01.004

(A) Hematoxylin and eosin stained liver sections from C57BL/6-splenectomized and C57BL/6 control mice (Top) and C57BL/6-f/f splenectomized and C57BL/6-f/f control mice (bottom) at indicated time points following a PHZ induced acute anemia. Examples of the darker staining erythropoietic clusters are shown in gray boxed area marked in the day 6 in C57BL/6 splenectomized and control mice and on day 8 in the C57BL/6-f/f splenectomized and control mice. Magnified views of the gray boxed regions are shown to the right of the panels. At least two independent livers were analyzed at each time point. Each liver was fixed, cut into several pieces and sectioned so that each section had fields from multiple areas of the liver. 2–3 sections per time point were analyzed and representative fields shown. (B) Schematic of liver acinus (functional interpretation of liver organization) formed from classical liver lobules. Blood enters the liver from portal triads composed of portal vein, hepatic artery and bile duct flows towards the central vein. This creates a gradient of oxygen concentration ranging highest in zone 1 (1), intermediate in zone 2 (2), to lowest in zone 3 (3). (C) Liver sections from C57BL/6-splenectomized and C57BL/6 control mice and (D) C57BL/6-f/f splenectomized and C57BL/6-f/f control mice recovering from an acute anemia stained with anti-BMP4 antibodies and HRP-conjugated secondary antibodies (BMP4) or secondary alone (control). At least two independent livers were analyzed at each time point. Each liver was fixed, cut into several pieces and sectioned so that each section had fields from multiple areas of the liver. 2–3 sections per time point were analyzed and representative fields shown. (*) denotes portal vein of triad composed of portal vein, hepatic artery and bile duct. Arrow points to a central vein. BMP4 staining is brown, with blue hematoxylin counter-stain. Note an example of how BMP4 expression remains expanded into zones 1 and 2 (bracket) into Day 6 post-PHZ in splenectomized mice, while it returns to exclusively zone 3 (3) in wild-type mice by 4 Days post anemia.

(A) Hematoxylin and eosin stained liver sections from C57BL/6-splenectomized and C57BL/6 control mice (Top) and C57BL/6-f/f splenectomized and C57BL/6-f/f control mice (bottom) at indicated time points following a PHZ induced acute anemia. Examples of the darker staining erythropoietic clusters are shown in gray boxed area marked in the day 6 in C57BL/6 splenectomized and control mice and on day 8 in the C57BL/6-f/f splenectomized and control mice. Magnified views of the gray boxed regions are shown to the right of the panels. At least two independent livers were analyzed at each time point. Each liver was fixed, cut into several pieces and sectioned so that each section had fields from multiple areas of the liver. 2–3 sections per time point were analyzed and representative fields shown. (B) Schematic of liver acinus (functional interpretation of liver organization) formed from classical liver lobules. Blood enters the liver from portal triads composed of portal vein, hepatic artery and bile duct flows towards the central vein. This creates a gradient of oxygen concentration ranging highest in zone 1 (1), intermediate in zone 2 (2), to lowest in zone 3 (3). (C) Liver sections from C57BL/6-splenectomized and C57BL/6 control mice and (D) C57BL/6-f/f splenectomized and C57BL/6-f/f control mice recovering from an acute anemia stained with anti-BMP4 antibodies and HRP-conjugated secondary antibodies (BMP4) or secondary alone (control). At least two independent livers were analyzed at each time point. Each liver was fixed, cut into several pieces and sectioned so that each section had fields from multiple areas of the liver. 2–3 sections per time point were analyzed and representative fields shown. (*) denotes portal vein of triad composed of portal vein, hepatic artery and bile duct. Arrow points to a central vein. BMP4 staining is brown, with blue hematoxylin counter-stain. Note an example of how BMP4 expression remains expanded into zones 1 and 2 (bracket) into Day 6 post-PHZ in splenectomized mice, while it returns to exclusively zone 3 (3) in wild-type mice by 4 Days post anemia.