Figure 1.

PMF of the DNA hairpin and dsDNA handles system 20R55∕4T under 14.19 pN external force. The PMF is computed directly from the data collected with that external force using the observed occupancy as described in the text (black filled squares) and also using the MBAR estimate with data from experiments from a range of external forces (unfilled gray diamonds), with error bars in the corresponding color. Note that in most cases, the MBAR error bars are less than the height of the corresponding symbol.