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. Author manuscript; available in PMC: 2010 Feb 15.
Published in final edited form as: Int J Cancer. 2009 Feb 15;124(4):772–777. doi: 10.1002/ijc.24052

Figure 1.

Figure 1

Skipping of exon 15 in HER2 pre-mRNA. A) SSOs were designed to target the 5’ splice site of exon 15. Skipping of exon 15 induced by SSOs generated a downstream stop codon, and results in a truncated HER2 ECD. Thick bar: SSO. Part of HER2 pre-mRNA (3’ to 5’, with intron underlined), SSO111, and SSO111m (with mismatches underlined and highlighted in bold) were shown in B). B) and C) Results from the analysis of total cellular RNA by RT-PCR are shown: B) Dose-dependence of SK-BR-3 cells treated with SSO111 at designated concentrations for 24 hours; C) Time-course of Sk-BR-3 cells treated with SSO111 for the designated time at 100 nM. The lengths of the PCR products (in base pairs) are indicated. Lower panels in B) and C): quantitative analysis of the results. Gray bars: SSO111 transfected cells; white bars: 111m-transfected cells.