Figure 1.

WOX11 Is Expressed in Cell Division Regions in Roots and Shoots.

(A) Detection of WOX11 transcript by RT-PCR in callus (Ca), roots (Ro), seedlings (Se), internodes (In), mature leaves (Le), flag leafs (Fl), and panicles (Pa). PCRs without prior reverse transcription (−RT) are included. Actin transcripts were detected as controls.

(B) to (I) In situ hybridization detection of WOX11 transcripts in the primary root with an antisense (B) or a sense probe (C), in crown root initials (D), in growing crown primordia ([E], longitudinal section; [F], transverse section), in a mature crown root (G), and in the SAM with an antisense (H) or a sense probe (I). Bars = 25 μm in (B), (C), (G), (H), and (I) and 50 μm in (D) to (F). Arrows indicate emerging crown roots.

(J) to (M) GUS activity in a 7-d-old WOX11p-GUS transgenic seedling: a primary root tip (J); lateral roots (K); and a transverse section of the root meristematic zone (L). (M) shows a transverse section through a lateral root.

(N) DR5-GUS expression profile in root. Bars = 25 μm in (J), (L), and (N) and 50 μm in (K) and (M).

(O) to (S) Detection of WOX11p-GUS expression during the course of crown root initiation. GUS activity was detected in emerging crown roots ([R] and [S]) but not at the early stages during crown root initiation ([O] to [Q]). Arrowheads indicate crown root initials. Bars = 25 μm.