Figure 10.

Arabidopsis MED21 Is Required for Resistance to A. brassicicola.

(A) and (B) Quantitative RT-PCR showing the expression of MED21 in transgenic MED21 RNAi (A) and 35S:MED21 (B) lines.

(C) to (E) Disease symptoms (C), size of disease lesion (D), and fungal growth (E) in MED21 RNAi and overexpression lines after A. brassicicola inoculation.

The quantitative RT-PCR data represent the mean ± se from three replicates. In (C), the data are the mean ± se from a minimum of 20 disease lesions. In (E), fungal growth in inoculated plants was determined using the amplification levels of A. brassicicola CutinA (Ab cutA) relative to the Arabidopsis Actin2 gene (At Actin). MO, MED21 overexpression lines; Mi, MED21 RNAi lines. In all cases, the experiments were repeated at least twice.