Figure 3.

HUB1 Regulates Flowering Time in Arabidopsis.

(A) Number of rosette leaves on plants grown under different photoperiods indicative of early flowering in hub1.

(B) Expression of flowering genes.

(C) and (D) Susceptibility of hub1 plants grown under short days to A. brassicicola (C) and B. cinerea (D).

In (A), analysis of variance was performed to determine the statistical significance of the differences between mean numbers of rosette leaves using SAS software (SAS Institute, 1999). Means with different letters are significantly different from each other (P = 0.05). In (B), quantitative RT-PCR was used to determine the expression of flowering genes relative to Actin2 gene. Experiments were repeated at least three times with similar results. d, days after inoculation.