Figure 4.

Coimmunoprecipitation of RGS9 and G_β5L expressed in the HEK293 cells. HEK293 cells were transfected with pcDNA3-RGS9 plasmid, pcDNA3-G_β5L and pEV1-G_γ2 plasmids, or all three plasmids. Membranes from each transfected line were solubilized in 0.5% lauryl sucrose and immunoprecipitated with rabbit anti-RGS9c antibodies. Three identical Western blots were performed with the aliquots of unbound proteins in the supernatant (S) and proteins bound to the pelleted beads (P). Each blot was probed with one of three antibodies: purified sheep anti-RGS9c (A), purified sheep anti-G_β5L NT_S antibodies (B), and commercial rabbit anti-G_γ2 antibodies (C).