Figure 2.

SLL1 Deficiency Results in Increased Chlorophyll Content.

(A) Distribution of mesophyll cells on the abaxial side of wild-type and sll1-1 leaves. The broader distribution of mesophyll cells on the abaxial side of sll1-1 leaves is highlighted (arrows), rendering the deeper green color of sll1-1 abaxial epidermis. Sections (∼20 μm) of rice leaf blades were stained in aniline blue solution. Bars = 20 μm (top panel) or 2 mm (bottom panel).

(B) Chlorophyll levels in wild-type, sll1-1, sll1-2, and sll1-2 plants with complemented expression of SLL1. To measure chlorophyll levels, we collected samples from an area from the middle part of the second leaf from the top of wild-type, sll11-1, sll1-2, or transgenic sll1-2 plants with complemented expression of SLL1 (at the same growth stage). Experiments are biological replicates. Error bar represents sd (n > 30 in each measurement), and statistical analysis by heteroscedastic t test indicates the significant differences (* P < 0.05; ** P < 0.01).