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. 2009 Mar;21(3):719–735. doi: 10.1105/tpc.108.061457

Figure 9.

Figure 9.

Enhanced Expression of SLL1 Stimulated Phloem Development in the Abaxial Cell Layer and Suppressed the Formation of Sclerenchymatous Cells in the Adaxial Cell Layer.

(A) qRT-PCR analysis confirmed the enhanced expression of SLL1 (top panel). The transgenic plants (3-week-old seedlings) revealed a phenotype with upper-rolled leaves (bottom panel, bar = 5 cm). The enlarged squared regions highlight leaf rolling compared with control. Experiments are biological replicates.

(B) Enhanced development of phloem of SLL1-overexpressing plants. Cross-section analyses were performed to analyze the development of phloem in the midrib (the squared regions are enlarged) and the large and small veins of wild-type and SLL1-overexpressing plants. P, phloem. Bars = 50 μm.

(C) Defective formation of sclerenchymatous cells in the adaxial cell layer of SLL1-overexpressing plants. Broken arrows indicate the regions where sclerenchymatous cells would have formed in normal leaf (bottom panel). Bars = 100 μm.