Figure 3.
P. syringae–Induced Leaf Expression of the BSMT1 Methyl Transferase Gene and Identification of Nonexpressing T-DNA Insertion Lines.
(A) Expression of BSMT1 in Col-0 leaves inoculated with Psm avrRpm1 (Psm avr) or Psm. Control samples were infiltrated with 10 mM MgCl2. Leaf samples were taken at 6, 10, and 24 HAI for RNA gel blot analysis.
(B) PCR analyses using genomic DNA from Col-0, bsmt1-1 (SALK_140496), and bsmt1-2 (WiscDSLox430E05) mutant plants as templates and primers specific for the BSMT1 gene sequence. The actin gene ACT2 was amplified as a control.
(C) Expression patterns of BSMT1 in Col-0 and bsmt1 leaves infiltrated with 10 mM MgCl2 or Psm avrRpm1 (Psm avr) as assessed by gel blot analysis. Leaf samples were taken at 10 and 24 HAI.