Figure 7.

Local Defense Responses in bsmt1 Plants Are Similar to Those in the Wild Type.

(A) and (B) Bacterial growth quantification of Psm avrRpm1 (OD = 0.005) (A) and Psm (OD = 0.002) (B) in leaves of wild-type and bsmt1 mutant plants 3 DAI. Bars represent means (±sd) of cfu per cm² from at least six parallel samples from different plants, each sample consisting of three leaf disks. No significant differences in bacterial numbers were detected at 3 DAI and 1 HAI (data not shown) for samples from different lines.

(C) and (D) Accumulation of the defense hormones SA (C) and JA (D) at sites of Psm avrRpm1 inoculation (10 HAI). Control samples were infiltrated with 10 mM MgCl₂.

(E) RNA gel blot analysis of PR-1 expression in Col-0 and bsmt1 leaves infiltrated with 10 mM MgCl₂ or Psm avrRpm1 (Psm avr). Leaf samples were taken at 10 and 24 HAI.

(F) Relative ICS1 expression in Col-0 and bsmt1 leaves infiltrated with 10 mM MgCl₂ or Psm avrRpm1, as assessed by quantitative real-time PCR analyses (see Figure 5D for details). Leaf samples were taken at 10 and 24 HAI. Asterisk indicates statistically significant differences between Psm avrRpm1–treated wild-type and mutant samples (P < 0.05).