P. syringae–Induced MeSA Formation but Not SAR Is Dependent on Bacterial Production of the Phytotoxin Coronatine.
(A) MeSA emission from Col-0 leaves after inoculation with coronatine-producing Pst, coronatine-deficient Pst cor−, and MgCl2 infiltration. Volatiles were sampled from 0 to 24 HAI, and mean values of ng emitted substance g−1 leaf FW h−1 (±sd, n = 7) are given. Different letters symbolize statistically significant differences between treatments (P < 0.002).
(B) SAR induction by Pst and Pst cor− in Col-0 plants. 1° leaves were infiltrated with MgCl2, Pst, or Pst cor− (OD 0.01 each), 2° leaves were challenge-infected 2 d later with Psm (OD 0.002), and quantities of Psm in 2° leaves were determined another 3 d later (see Figure 5C for details). Bars represent means (±sd) of cfu per cm2 from at least six parallel samples. Different characters symbolize statistically significant differences between treatments (P < 0.01).