Table 2.
Fold-Changes of Transcript Levels of Genes Involved in GL Biosynthetis and Its Transcriptional Regulation in apk1 apk2 Plants
| Microarrays
|
qRT-PCR
|
||||||
|---|---|---|---|---|---|---|---|
| Gene | AGI | Description | Fold-Change | P | q | Fold-Change | P |
| APK1 | At2g14750 | APS kinase 1 | 0.0111 | 0.0000002 | 0.00006 | 0.0017 | 0.0004 |
| APK2 | At4g39970 | APS kinase 2 | 0.0294 | 0.00493 | 0.0805 | 0.0013 | 0.0002 |
| APK3 | At3g03900 | APS kinase 3 | 1.070 | 0.568 | 0.532 | 1.147 | 0.0751 |
| APK4 | At5g67520 | APS kinase 4 | 0.548 | 0.233 | 0.418 | 0.911 | 0.665 |
| MAM1 | At5g23010 | Methylthioalkylmalate synthase 1 | 3.13 | 0.000045 | 0.0058 | ||
| MAM3 | At5g23020 | Methylthioalkylmalate synthase 3 | 8.318 | 0.0000073 | 0.0018 | 18.089 | 0.0083 |
| ASA1 | At5g05730 | Anthranilate synthase α-subunit | 1.98 | 0.0170 | 0.142 | ||
| ASB1 | At1g25220 | Anthranilate synthase β-subunit | n/r | – | – | ||
| TSA1 | At4g02610 | Tryptophan synthase α-subunit 1 | 1.24 | 0.0157 | 0.137 | ||
| TSB1 | At5g54810 | Tryptophan synthase β-subunit 1 | n/r | – | – | 4.673 | 0.0073 |
| CYP79A2 | At5g05260 | CYTOCHROME P450 79A2 | n/e | – | – | ||
| CYP79F1/SUS1 | At1g16410 | CYTOCHROME P450 79F1, SUPERSHOOT1 | n/r | – | – | ||
| CYP79F2 | At1g16400 | CYTOCHROME P450 79F2 | n/r | – | – | ||
| CYP79B2 | At4g39950 | CYTOCHROME P450 79B2 | 8.524 | 0.000125 | 0.0121 | ||
| CYP79B3 | At2g22330 | CYTOCHROME P450 79B3 | 4.967 | 0.000268 | 0.0201 | ||
| CYP83A1 | At4g13770 | CYTOCHROME P450 83A1 | 2.313 | 0.000389 | 0.0236 | ||
| CYP83B1/SUR2 | At4g31500 | CYTOCHROME P450 83B1, SUPERROOT2 | 1.918 | 0.000362 | 0.0229 | 4.062 | 0.0083 |
| SUR1 | At2g20610 | C-S lyase, SUPERROOT1 | 2.076 | 0.000212 | 0.0169 | 4.303 | 0.0150 |
| UGT74B1 | At1g24100 | UDP-Glucosyltransferase 74B1 | 2.389 | 0.00246 | 0.0598 | ||
| UGT74C1 | At2g31790 | UDP-Glucosyltransferase 74C1 | 2.225 | 0.0000785 | 0.0132 | ||
| SOT16 | At1g74100 | Sulfotransferase, indolic GLs | 2.097 | 0.00489 | 0.0805 | 4.624 | 0.0082 |
| SOT17 | At1g18590 | Sulfotransferase, aliphatic GLs | 4.186 | 0.000146 | 0.0130 | 7.636 | 0.0075 |
| SOT18 | At1g74090 | Sulfotransferase, aliphatic GLs | 2.291 | 0.000278 | 0.0239 | 5.648 | 0.0120 |
| At1g65880 | Benzoate-CoA ligase | n/e | – | – | |||
| AOP1 | At4g03070 | 2-Oxoglutarate-dependant dioxygenase | 1.09 | 0.476 | 0.494 | ||
| AOP2 | At4g03060 | 2-Oxoglutarate-dependant dioxygenase | 3.15 | 0.000150 | 0.0132 | ||
| AOP3 | At4g03050 | 2-Oxoglutarate-dependant dioxygenase | n/e | – | – | ||
| HAG1/MYB28 | At5g61420 | High Aliphatic GL 1 R2R3-MYB transcription factor | 1.326 | 0.0185 | 0.146 | 2.094 | 0.0405 |
| HAG2/MYB76 | At5g07700 | High Aliphatic GL 2 R2R3-MYB transcription factor | 7.694 | 0.0000208 | 0.0365 | 9.324 | 0.009 |
| HAG3/MYB29 | At5g07690 | High Aliphatic GL 3 R2R3-MYB transcription factor | 2.370 | 0.0277 | 0.178 | 3.591 | 0.0096 |
| HIG1/MYB51 | At1g18570 | High Indolic GL 1 R2R3-MYB transcription factor | 1.325 | 0.060 | 0.246 | 2.000 | 0.0466 |
| HIG2/MYB122 | At1g74080 | High Indolic GL 2 R2R3-MYB transcription factor | n/e | – | – | 3.06 | 0.0352 |
| HIG3/MYB34/ ATR1 | At5g60890 | High Indolic GL 3 R2R3-MYB transcription factor | 15.796 | 0.116 | 0.312 | 3.205 | 0.0357 |
| OBP2 | At1g07640 | DOF transcription factor | 0.870 | 0.222 | 0.410 | ||
| IQD1 | At3g09710 | Calmodulin binding protein | 0.610 | 0.374 | 0.450 | ||
| FRY1/SAL1 | At5g63980 | Fiery1/Sal1, 3′(2′),5′-bisphosphate nucleotidase activity; detox of PAP | 2.044 | 0.000453 | 0.0256 | ||
n/e, not expressed; n/r, not represented on the ATH1 microarray. Transcripts highlighted in bold are significantly altered in the apk1 apk2 mutant compared with Col-0 samples at q < 0.05 or by qRT-PCR at P < 0.01.