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Light microscopic observation on the alternative 3D-in vitro model utilizing HaCaT cells cultured without fibroblasts (A) and air-exposed co-cultured system in the presence of middle ear cholesteatoma fibroblasts (B). HaCaT cells show bigger stratification when co-cultured with fibroblasts. Hematoxylin-eosin staining, Original magnification ×400.
