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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Immunol. 2009 Mar 1;182(5):3055–3062. doi: 10.4049/jimmunol.0802749

Figure 6. Increased Production of DC chemoattractants by Colonic Epithelial Cells in Resistant Mice Promote DC recruitment.

Figure 6

Epithelial cells (n=4) from the proximal colons of resistant BALB/c, susceptible AKR and susceptible BALB/c mice were analysed for expression of CCL2, CCL3, CCL5, CCL20 and TSLP mRNA 0, and 24 hours (A) and 7 days post-infection (B) by q-PCR. The data is shown as the relative expression of mRNA compared to samples from naïve non-infected animals. (C) In order to assess whether CCL5 and CCL20 were necessary for DC recruitment in resistant BALB/c mice, BALB/c mice were infected with T.muris and given intravenous neutralising antibodies to CCL5 and CCL20 (left hand column) or control rat IgG (right hand column). After 24 hours the DCs were enumerated by flow cytometry. In antibody treated mice there was a complete ablation of DC recruitment (***p=0.0001, n=8).