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. Author manuscript; available in PMC: 2010 Apr 10.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Feb 15;381(3):393–396. doi: 10.1016/j.bbrc.2009.02.056

Figure 1. Proteomics-Based Screen for ROCKII Interactors.

Figure 1

1A. 1D SDS PAGE and Coomassie staining of eluted proteins from a control column and ROCKII affinity column shows strong enrichment for protein binding to the affinity column over the control column. 1B. 2D SDS-PAGE of eluted proteins from both control and affinity columns facilitated spot-picking prior to mass spectrometry analysis and protein identification. 1C. Listing of numbers of proteins isolated and identified via MALDI TOF analysis.