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. Author manuscript; available in PMC: 2009 Apr 22.
Published in final edited form as: Nat Med. 2008 Jun 1;14(6):667–675. doi: 10.1038/nm1775

Figure 2.

Figure 2

The regulation of adipose tissue lipogenesis by MBH leptin is STAT3 independent. (a) Western blot analysis of WAT protein extracts derived from clamped rats. (b) Fold change in mRNA levels of the SREBP1c gene targets Acaca, Fasn and Scd1 after treatment with leptin, leptin plus STAT3 PI or a vehicle control in WAT as determined by real-time PCR. mRNA levels of each gene were normalized to their respective18S RNA levels and then normalized again to the level of the vehicle control. (c,d) Fold change in mRNA levels in WAT after treatment with leptin, leptin plus STAT3 PI or a vehicle control of Srebf, Pparg and its target gene Lpl and the lipases Pnpla2 and Lipe (c) and of Pck, the rate-limiting enzyme of glyceroneogenesis (d). *P < 0.05 versus MBH vehicle–treated group.