Figure 7.

Reduced [³H]Tdr is due to the low S-phase fraction in ChoK downregulated breast cancer cells. MDA-MB-231 (clear bar), MCF-7 (gray bar), and MCF-12A (solid black bar) breast cancer cell lines were untreated, treated with Oligofectamine alone, or transfected with ChoK-specific siRNA. At 48 hours after transfection, cells were incubated with BrdU for 45 minutes and analyzed by flow cytometry after S-phase fraction staining with anti-BrdU and DNA staining with PI as indicated in the Materials and Methods section. Results shown are the mean values of single experiment performed in triplicate and from three independent experiments. The significance of the value is indicated by asterisks (*), and the comparative reference is the corresponding cell line treated with Oligofectamine alone. ns indicates nonsignificant. *P < .05, **P < .01, ***P < .001.