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. 2009 May;11(5):497–508. doi: 10.1593/neo.81618

Figure 6.

Figure 6

Quantification of VEGF and endostatin secretion in pancreatic cancer-stellate cell coculture SNs by ELISA: PSC (200,000 cells per well) and PCC (400,000 cells per insert, 1-µm pore size) were cocultured for 24 hours in serum-free medium. The amount of VEGF (A) and endostatin (C) secreted in the common SN was quantified by ELISA. The experiments were repeated at least twice using different PSC clones. In comparison to the mathematical sum of the individual controls (PCC + PSC), the increase of VEGF (B) and endostatin (D) in the common SN after coculture of MiaPaCa-2 and Panc-1 with PSCs is expressed in percentages.