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. Author manuscript; available in PMC: 2010 Apr 7.
Published in final edited form as: Biochemistry. 2009 Apr 7;48(13):2981–2989. doi: 10.1021/bi802295z

Figure 5.

Figure 5

Stopped-flow kinetic studies on Cu-L1. (A) Stopped-flow fluorescence trace of the reaction of 50 μM Cu-L1 and 50 μM nitrocefin at 10 °C. (B) Stopped-flow UV-Vis traces of the reaction of 50 μM Cu-L1 with 50 μM nitrocefin at 10 °C. The absorbance at 485 nm is due to the product, the absorbance at 390 nm is due to the substrate, and the absorbance at 665 nm is due to the intermediate.