Table 1.

Effect of T₃ treatment on transporter expression in cultured granular cells

Transporter mRNA	No T3		2.5 nm T3		5.0 nm T3
	Wt	KO	Wt	KO	Wt	KO
Mct8	591 ± 130		596 ± 48		621 ± 109
Oatp2	27.0 ± 9.1	28.0 ± 8.5	29.3 ± 5.4	23.9 ± 6.1	28.3 ± 1.6	19.5 ± 4.7
Oatp14	7.4 ± 3.4	6.8 ± 2.4	4.9 ± 0.9	2.8 ± 0.4	5.9 ± 1.7	2.5 ± 0.3a

Primary cultures of granular cells from the cerebella of Wt and Mct8 KO mice were incubated in the presence of 0, 0.2, 0.5, 1.25, 2.5, and 5.0 nm T₃ for 24 h. Expression of Mct8 (Slc16a2), Oatp2 (Slco1a4), and Oatp14 (Slc1c1) was quantified by real-time PCR using TaqMan probes. Shown are the data (mean number of RNA copies relative to 18S RNA ± sd) from cells incubated without added T₃ or in the presence of 2.5 and 5.0 nm only. Mct8 mRNA was not detected in the KO cells. The cells used in this experiment are the same as for Hr mRNA quantification shown in Fig. 4. Two-way ANOVA using the data from all T₃ concentrations revealed that there was no difference of genotype or treatment, except for the highest T₃ dose that decreased Oatp14 mRNA in the KO cells. 

^aP < 0.05.