Figure 4.

Kisspeptin (Kiss) inhibits the baclofen (Bac)-induced outward current and reverses the hyperpolarization of GnRH neurons from both oil- and E2-treated animals. A and B, Representative recordings show that kisspeptin-10 inhibited the baclofen-induced outward current in oil- (A) and E2 (B)-treated animals. C, Summary of the effects of kisspeptin-10 (10 nm) on baclofen (3 μm)-induced outward current as shown in A and B. **, P < 0.01 and ***, P < 0.001 (t test), baclofen responses with coperfusion of kisspeptin-10 compared with baclofen only (control). Cell numbers are indicated. Con, Control. D–F, Representative recordings showing that kisspeptin-10 reversed the baclofen-induced hyperpolarization at even lower concentrations in both E2- (D) and oil- (E and F)-treated animals. The initial resting membrane potentials are indicated.