Skip to main content
. 2008 Dec 23;150(5):2395–2403. doi: 10.1210/en.2008-1027

Figure 2.

Figure 2

PR binds to the endogenous LHβ promoter in LβT2 cells, and the DNA-binding domain is necessary for progesterone suppression of LHβ gene expression. A, ChIP was performed using cross-linked protein/chromatin from LβT2 cells treated with vehicle (Veh) or R5020 and antibodies directed against PR or nonspecific IgG as a negative control. PCR primers encompassing the proximal promoter of LHβ were used to detect precipitation of genomic DNA. PCR amplification was performed on 0.2% chromatin input (lanes 1 and 2), and chromatin was precipitated with either mouse IgG (lanes 3 and 5) or PR antibody (lanes 4 and 6). B, The −1800-bp LHβ-luc reporter plasmid was cotransfected with wild-type PRB or PRB DBD mutant (PRC577A). C, The −1800-bp LHβ-luc reporter plasmid was cotransfected with mouse PRB or PRA. After overnight starvation in serum-free media, the cells were treated with vehicle, 100 nm R5020, 10 nm GnRH, or both hormones as indicated. *, Significant differences from the vehicle-treated control; †, interaction as defined by a two-way ANOVA (P < 0.05). luc, Luciferase.