Figure 4.

The effects of insulin and leptin on liver apoB-100 and apoB-48. Fasted (18 h) rats fed a standard chow diet received a continuous iv infusion of vehicle, insulin, leptin, or combined leptin and insulin as described in Materials and Methods. Subsequently liver was taken and apoB-100 and apoB-48 were measured by immunoblot as described in Materials and Methods. Autoradiograph composites from each experimental condition for apoB-100 and apoB-48 and tubulin are presented and the quantification of the total data for each condition is presented below each autoradiograph (n = minimum of six animals/group). Results are presented as mean ± se. Significant differences are indicated. A, Vehicle vs. insulin; B, insulin vs. leptin/insulin; C, vehicle vs. leptin. LIV, Liver.