Figure 6.

The effects of insulin and leptin on liver ACC phosphorylation and fatty acid oxidation. Fasted (18 h) rats fed a standard chow diet received a continuous iv infusion of vehicle, insulin, leptin, or combined leptin and insulin as described in Materials and Methods. Subsequently liver was taken and ACC phosphorylation was measured by immunoblot (A) or fatty acid oxidation was assessed (B) as described in Materials and Methods. For phospho (p) and total (t) ACC, autoradiograph composites from each experimental condition are presented and the quantification of the total data for each condition is presented below each autoradiograph. B, n = minimum of six animals/group. Results are presented as mean ± se. Significant differences are indicated. Lep, Leptin; Ins, insulin.