Table 1.
Summary of the yeast and bacterial expression plasmids used in this study
| Protein product | Reference | |
|---|---|---|
| Yeast expression plasmids | ||
| pRS316 (CEN/URA3) | Sikorski and Hieter, 1989 | |
| pRS316-CUE1 | Cue1p | This study |
| pRS316-cue1AVA | Cue1pAVA | This study |
| pRS316-cue1MFP | Cue1pMFP | This study |
| pRS316-cue1ΔCD | Cue1pΔCD | This study |
| pRS316-cue11-173 | Cue1p1-173 | This study |
| pRS314 (CEN/TRP1) | Sikorski and Hieter, 1989 | |
| pRS314-CUE1 | Cue1p | This study |
| pRS314-cue1ΔCD | Cue1pΔCD | This study |
| pRS314-cue11-173 | Cue1p1-173 | This study |
| pRS314-cue124-203 | Cue1p24-203 | This study |
| pRS314-cue1110-203 | Cue1p110-203 | This study |
| pRS314-cue1151-203 | Cue1p151-203 | This study |
| pRS424 (2 μ/TRP1) | Sikorski and Hieter, 1989 | |
| pRS424-GFPcue1110-203 | GFP-Cue1p110-203 | This study |
| pRS424-GFPcue1151-203 | GFP-Cue1p151-203 | This study |
| pRS316-UBC7HA | Ubc7pHA | This study |
| pRS316-™ UBC7HA | ™Ubc7pHA | This study |
| pCTG* | CTG* | Kostova and Wolf, 2005 |
| pSM1911 | Ste6p*HA | Huyer et al., 2004 |
| Bacterial expression plasmids | ||
| pGEX-4T-1 | GST | Amersham Biosciences |
| pGEX-4T-1cue125-203 | GST-Cue1p25-203* | This study |
| pGEX-4T-1cue125-173 | GST-Cue1p25-173* | This study |
| pGEX-4T-1cue1110-203 | GST-Cue1p110-203 | This study |
| pGEX-4T-1cue1151-203 | GST-Cue1p151-203* | This study |
| pGEX-4T-1cue1165-203 | GST-Cue1p165-203 | This study |
| pGEX-4T-1cue1175-203 | GST-Cue1p175-203 | This study |
| pGEX-4T-1cue1151-191 | GST-Cue1p151-191 | This study |
| pGEX-4T-1cue125-ΔCD-203 | GST-Cue1p25-ΔCD-203* | This study |
| pGEX-4T-1hrd1207-551 | GST-Hrd1pC | This study |
| pGEX-4T-1UBC7 | GST-Ubc7p* | This study |
| pDEST47-UBC7 | Ubc7p | This study |
| pGEX-KG-UBCH5b | GST-UbcH5b | This study |
Letters and numbers shown in superscript, indicate amino acid name and positions in Cue1p. Protein products denoted with an asterisk were used both in their GST-fused and thrombin-cleaved forms.