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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6082–6091. doi: 10.4049/jimmunol.181.9.6082

Figure 1.

Figure 1

Combined crosslinking of CD3 and CD4 induces tyrosine phosphorylation independently of CD45. (A) NP-40 soluble cell lysates of total thymocytes (2 × 106) from CD45+/− or CD45−/− mice expressing polyclonal TCR were analyzed for total tyrosine phosphorylation (pY) by western blot following stimulation with either CD3 and/or CD4. The pY blot was then stripped and probed for phosphorylated ERK (pERK) and repeated again for total ERK. The data shown are representative of two independent experiments.