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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Immunol. 2008 Nov 1;181(9):6082–6091. doi: 10.4049/jimmunol.181.9.6082

Figure 6.

Figure 6

Defect in CD69 induction in CD45 deficient thymocytes. (A) Representative dot plots displaying CD69 expression gated on CD4+CD8+ population after overnight stimulation with 5μg/mL pMCC and/or PMA at 0.1ng/mL. (B) Graph of %CD69 expression from flow cytometry data of thymocytes from CD45+/− or CD45−/− AND TCR transgenic mice after overnight stimulation in the presence of T cell depleted spleenocytes with varied concentrations of peptide (serial 10 fold dilutions with highest concentration at 5 μg/ml) and/or PMA at 0.1 ng/mL. All CD69 expression data presented is gated on CD4+CD8+Vα11+ population. This data is representative of three independent experiments.