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. 2009 Feb 17;18(10):1719–1739. doi: 10.1093/hmg/ddp075

Figure 10.

Figure 10.

Gli2 and Gli3 expression and Gli3 processing are not altered in hitchhiker mutants. (A) Quantitative RT–PCR analysis with primers for Gli2 and Gli3 on RNA from caudal ends of E9.5 wild-type, heterozygous and hitchhiker mutant embryos, showing no change in expression. (B) Western blot analysis on total cell lysates from E9.5 limb buds demonstrate no apparent change in abundance of the full length 190 kDa Gli3 isoform (Gli3FL) nor the 83 kDa cleaved repressor isoform (Gli3R), in hitchhiker embryos compared with wild-type or heterozygous littermates. Western blots with extracts from Gli3/+ and Gli3/Gli3 mutants confirm correct Gli3 band identify. β-Tubulin was used as a loading control. (C) Western blots with nuclear extracts from E10.5 caudal ends demonstrate similar abundance of the 190 and 83 kDa Gli3 isoforms in hitchhiker embryos compared with wild-type or heterozygous littermates; histone H1 was used as a loading control.