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. 1999 Mar 2;96(5):1965–1970. doi: 10.1073/pnas.96.5.1965

Figure 1.

Figure 1

Cloning strategy, schematic view of the crayfish CP cDNA. The obtained CP cDNA with positions indicated for the ORF (thick, black line), start codon (159 ATG), and stop codon (5322 TAG). Nucleotides 115-6265 were first obtained by repeated PCRs, labeled PCR cDNA in the figure. Radioactively labeled PCR products from different parts of the PCR cDNA (probes I–VI) were used to screen the cDNA library by plaque hybridization. The selected clones from the cDNA library screening that were sequenced are indicated as I-1, II-1, III-5, VI-20, and VI-19. Roman numerals indicate which probe was used to obtain the clones. F1 and F2 are PCR products obtained from first-strand cDNA, covering sequences that are not present in any of the cDNA clones.