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. 2009 Mar 2;10(3):906–928. doi: 10.3390/ijms10030906

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Intracellular distribution of Hap46/BAG-1M and Hap50/BAG-1L. (A and B) HeLa cells were transfected with a construct in which the sequence encoding Hap46/BAG-1M was coupled to that for the green fluorescent protein GFP. In A, cells were maintained at 37°C, in B they were exposed to a 42°C treatment for 2 h. Analysis was by fluorescence microscopy. From ref. [13]. (C) HeLa cells, similarly transfected with a construct coding for Hap50/BAG-1L coupled to GFP, were maintained at 37°C. In contrast to A and B, the cell body and the contours are practically invisible due to the very strong fluorescence originating from the nucleus. From ref. [14].