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. 2009 Mar 2;10(3):906–928. doi: 10.3390/ijms10030906

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Formation of Hap46/BAG-1M complexes with several transcription factors. Transcription factors, as indicated, were synthesized as radiolabeled proteins by in vitro transcription/translation from recombinant constructs. They were then incubated in the presence of hsc70, contained in the reticulocyte lysate, with Sepharose matrices to which either Hap46/BAG-1M (lanes 1) or deletion variant Hap46/BAG-1MΔC47 (lanes 2) had been attached. After extensive washing, retained proteins were eluted and analyzed by electrophoresis in SDS-containing polyacrylamide gels and radioautography. Glucocorticoid receptor (GR); Estrogen receptor (ER). From ref. [53].