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. 2009 Mar 16;10(3):1215–1225. doi: 10.3390/ijms10031215

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 2. — Effects of ultra low-dose γ-radiation using in-house fabricated radiation source on mRNA expression level of marker genes in leaves of rice seedlings by RT-PCR. (A) Leaf segments were prepared as described in Figure 1, pooled together after treatment for total RNA isolation followed by quality check, cDNA synthesis, and RT-PCR analysis. The gene specific primers used are provided in Supplementary Table 1. Leaf segments were also sampled from dummy (C: control), and whole plants (WP: whole plant control) that served as a positive control for background radiation in the room where the experiment (in an incubator) was carried out. (B) The relative intensity of mRNA level of each transcript in (A) are presented. The intensities were calculated by the ATTO image analysis software (ATTO, Tokyo, Japan). Results are the representative of two independent experiments.