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. 1999 Mar 2;96(5):1971–1976. doi: 10.1073/pnas.96.5.1971

Figure 4.

Figure 4

Expression of homogeneous rRNA in Δ7 prrn strains. 16S rRNA molecules in the rrn+ strain and in Δ7 prrn strains carrying either wt (pHK-rrnC+) or mutant (pSTL102) rRNA plasmid were analyzed by primer extension. DNA primers annealed to 16S rRNA molecules were extended in the presence of one dideoxynucleotide (ddATP) and three deoxynucleotides (dGTP, dCTP, and dTTP). Because mutant 16S rRNA produced from pSTL102 contains a C to U change, primers hybridized to wt and mutant 16S molecules are extended by four nucleotides and one nucleotide, respectively.