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. 1999 Mar 2;96(5):1971–1976. doi: 10.1073/pnas.96.5.1971

Figure 5.

Figure 5

Expression of foreign rRNA in Δ7 prrn strains. (A) Primer extension analysis. 23S rRNA molecules in the rrn+ strain and in Δ7 prrn strains carrying either E. coli (pSTL102) or S. typhimurium (pSt1-Km) rRNA plasmid were analyzed by primer extension as described in Fig. 4. The primer used in this experiment hybridizes to a common sequence in E. coli and S. typhimurium 23S rRNAs but gives different extension products because of a sequence difference between the two molecules. (B) Fragmentation of 23S rRNA. Total cellular RNA prepared from the rrn+ strain and Δ7 prrn strains carrying plasmids with E. coli (Ec, pSTL102), S. typhimurium (St, pSt1-Km), or P. vulgaris (Pv, pPM2) rRNA is shown. Fragmented 23S rRNA molecules are indicated by a prime.