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. Author manuscript; available in PMC: 2009 Apr 22.
Published in final edited form as: Plant Cell Physiol. 2004 Nov;45(11):1669–1680. doi: 10.1093/pcp/pch189

Fig. 4.

Fig. 4

Pn-AMP1 induces rapid depolarization of the actin cytoskeleton. BWG7a cells were incubated with 7.5 μM Pn-AMP1 for the indicated times, fixed with formaldehyde and then stained with Rh-Ph and DAPI to visualize the actin cytoskeleton and nuclei, respectively. (A) The percentages of small-budded cells exhibiting loss of actin cables or depolarization of actin patches were determined as described in Materials and Methods. n-values for each point were 130–270 cells. Error bars are the SEP. (B) Representative images of cells. Cells were observed by Nomarski microscopy (left), by fluorescence microscopy of DAPI staining to detect nuclei (center) and with Rh-Ph to detect F-actin (right). Scale bar is 5 μm.

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