Fig. 1.
Examples of responses of staphylococci to deferoxamine. Staphylococcus aureus KCTC1927, Staphylococcus epidermidis KCTC1917, Staphylococcus saprophyticus KCTC3345 and a coagulase-negative staphylococcus (CoNS: S. epidermidis) strain were preconditioned by culturing in BHI broth containing 200 µM of dipyridyl at 37℃ overnight to adapt to iron-restricted conditions and to reduce intracellular iron stores. About 103-4 cfu of these three strains were spread onto the surface of SSD agar containing 1 µM FeCl3, and then discs containing 30 µL of phosphate-buffered saline (PBS) or 100 µM deferoxamine were placed on the agar surface. S. aureus KCTC1927 was incubated at 37℃ for 24 hr, and S. epidermidis KCTC1917, S. saprophyticus KCTC3345 and the CoNS strain for 48 hr.