Fig. 4.

Utilization of transferrin-bound iron by staphylococci in the absence or presence of deferiprone. During culture in SSD broth containing holotransferrin (HT) or in SSD broth containing HT plus deferiprone (DFP), as shown in Fig. 3, culture supernatants were obtained by centrifuging culture aliquots at 10,000 rpm for 5 min at appropriate times. Equal volumes (20 µL) of culture supernatants were electrophoresed on 6 M urea-polyacrylamide gels, and stained with Coomassie blue. AP, MC, MN, and DF indicate apoferric, C-terminal monoferric, N-terminal monoferric and diferric transferrins, respectively.