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. 1999 Mar 2;96(5):1983–1988. doi: 10.1073/pnas.96.5.1983

Figure 4.

Figure 4

Thermal denaturation of the multiple-alanine mutants (10 μM) in buffer S. Normalized ellipticity is calculated as (ɛ − ɛ25)/(ɛ95 − ɛ25) where ɛ, ɛ25, and ɛ95 are the CD ellipticities at 222 nm at any temperature, 25°C, and 95°C, respectively.