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Heterodimerization assay; ability of 6His-tagged multiple-alanine mutants to bind untagged, wild-type Arc to Ni-NTA agarose
| 6His-tagged protein | Fraction untagged Arc in
|
|
|---|---|---|
| Ni-NTA flow-through + wash | Ni-NTA imidazole elution | |
| 3A | 0.89 | 0.11 |
| 7A | 0.78 | 0.22 |
| 11A | 0.73 | 0.27 |
| 12A | 0.66 | 0.34 |
| 15A | 0.56 | 0.44 |
| λN-domain-H6 | 1.00 | 0.00 |
| None | 0.99 | 0.01 |
