Figure 1.

Diagrams of the methods used for the collection of mainstream and sidestream cigarette smoke. The standard puff protocol: a hand held syringe was used to draw puffs of smoke (30 mL puff x 3 sec duration at 1 min. intervals, approx. 10 puffs per cigarette). Tar was collected on Cambridge filters. The second diagram shows the method used for the collection of the tar and gas-phase of sidestream cigarette smoke. The gas-phase (after retaining tar with a filter) was bubbled through 10 mL of the spin-trap PBN in benzene.