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. 1999 Mar 2;96(5):1995–2000. doi: 10.1073/pnas.96.5.1995

Figure 3.

Figure 3

RARα/RXRα heterodimers activate transcription from chromatin templates in a ligand- and template-specific manner. (A) tRA-induced derepression of transcription from chromatin templates by RARα/RXRα. In vitro transcription was performed on chromatin or naked (DR5)5β2G templates (200 pM) by using a HeLa cell nuclear extract (100 μg) for 45 min in the presence or absence of FhRARα/HmRXRα (1 nM) and tRA (1 μM) in a final reaction volume of 50 μl as indicated. S1 nuclease analysis was carried out after deproteinization. (B) Template specificity of activated transcription. Activation of transcription on chromatin (DR5)5β2G or (17M)5β2G templates was determined in the presence of 1 nM of activator [either Gal4(1–147), Gal4-VP16, or FhRARα/HmRXRα] with or without tRA (1 μM) as above. S1 nuclease digestion of RNA transcripts originating from β2G and pG1 templates generated 179- and 60-nt fragments, respectively (see Fig. 1).