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. 2009 Apr 2;106(16):6772–6777. doi: 10.1073/pnas.0811331106

Fig. 2.

Fig. 2.

Interaction of the MCH1R with D1R and D2R in the NAcSh. (A) Changes in NAcSh spike firing induced by MCH (2.5 μM) alone, D1 agonist (SKF 81297 3 μM) plus MCH, D2 agonist (Quinpirole 3 μM) plus MCH, D1+D2 agonists, D1+D2 agonists plus MCH, and D1+D2 agonists plus MCH and TPI 1361-17 (2 μM) (*, P < 0.05 for MCH/D1/D2 vs. each other condition, F (5, 25) = 6.66; *, P < 0.001, for 1-way ANOVA, followed by Bonferroni multiple comparison test; n = 4–6). Resting potential for MCH example was −82.4 and −83.2 mV before and after MCH, and resting potential for MCH/D1/D2 example was −80.2 and −79.6 mV before and after agonists. (B) Phosphorylation of DARPP-32 (Thr 34) in NAcSh slices after MCH (2.5 μM) alone, D1+D2 agonists (SKF 81297 3 μM and Quinpirole 3 μM), and D1+D2 agonists plus MCH (**, P < 0.01 for MCH/D1/D2 vs. control, ANOVA followed by Bonferroni multiple comparison test; n = 6–10). Immunoblots for detection of phospho-Thr-34 DARPP-32 are shown at the top. The levels of phospho-Thr-34 DARPP-32 were normalized to values obtained from control slices (a.u., arbitraty unit).